Abstract
Na(x) is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Na(x) has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Na(x) by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Na(x) in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Na(x) or depletion of SAP97 resulted in a decrease in cell-surface Na(x), which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Na(x) channels at the plasma membrane.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain / cytology
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Brain / metabolism*
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Cell Line, Tumor
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Cell Membrane / genetics
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Cell Membrane / metabolism*
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Discs Large Homolog 1 Protein
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Fluorescent Antibody Technique
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Gene Expression*
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Gene Silencing
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Guanylate Kinases / chemistry
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Guanylate Kinases / genetics
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Guanylate Kinases / metabolism*
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HEK293 Cells
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Humans
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Immunoprecipitation
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In Situ Hybridization
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Male
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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PDZ Domains
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Plasmids
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RNA, Small Interfering / genetics
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Subfornical Organ / cytology
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Subfornical Organ / metabolism*
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Transfection
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Voltage-Gated Sodium Channels / chemistry
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Voltage-Gated Sodium Channels / genetics
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Voltage-Gated Sodium Channels / metabolism*
Substances
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Discs Large Homolog 1 Protein
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Dlg1 protein, mouse
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Membrane Proteins
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RNA, Small Interfering
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Scn7a protein, mouse
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Voltage-Gated Sodium Channels
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Guanylate Kinases