We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.
Keywords: (090.1995) Digital holography; (110.0110) Imaging systems; (170.1530) Cell analysis; (170.1650) Coherence imaging; (320.7160) Ultrafast technology.