Genetic deletion of HRP2 and HRP3 in Indian Plasmodium falciparum population and false negative malaria rapid diagnostic test

Navin Kumar; Veena Pande; R M Bhatt; Naman K Shah; Neelima Mishra; Bina Srivastava; Neena Valecha; Anupkumar R Anvikar

doi:10.1016/j.actatropica.2012.09.015

Genetic deletion of HRP2 and HRP3 in Indian Plasmodium falciparum population and false negative malaria rapid diagnostic test

Acta Trop. 2013 Jan;125(1):119-21. doi: 10.1016/j.actatropica.2012.09.015. Epub 2012 Oct 3.

Authors

Navin Kumar¹, Veena Pande, R M Bhatt, Naman K Shah, Neelima Mishra, Bina Srivastava, Neena Valecha, Anupkumar R Anvikar

Affiliation

¹ National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi, India. [email protected]

PMID: 23041541
DOI: 10.1016/j.actatropica.2012.09.015

Abstract

Genetic polymorphisms in diagnostic antigens are important factors responsible for variable performance of rapid diagnostic tests. Additionally, the failure of antigen expression due to gene deletion may also contribute to variable performance. We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes leading to false negative results of rapid diagnostic tests. The study highlights need to determine the prevalence of P. falciparum isolates lacking these genes in larger field populations in India.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Protozoan / analysis*
Antigens, Protozoan / genetics
Diagnostic Tests, Routine*
False Negative Reactions*
Gene Deletion
Humans
Immunoassay / methods
India
Malaria, Falciparum / diagnosis*
Malaria, Falciparum / parasitology
Parasitology / methods*
Plasmodium falciparum / immunology
Plasmodium falciparum / isolation & purification*
Protozoan Proteins / analysis*
Protozoan Proteins / genetics

Substances

Antigens, Protozoan
HRP-2 antigen, Plasmodium falciparum
HRP3 protein, Plasmodium falciparum
Protozoan Proteins