Screening of lactic acid bacteria for reducing power using a tetrazolium salt reduction method on milk agar

Damien Michelon; Sybille Tachon; Bruno Ebel; Joëlle De Coninck; Gilles Feron; Patrick Gervais; Mireille Yvon; Rémy Cachon

doi:10.1016/j.jbiosc.2012.09.010

Screening of lactic acid bacteria for reducing power using a tetrazolium salt reduction method on milk agar

J Biosci Bioeng. 2013 Feb;115(2):229-32. doi: 10.1016/j.jbiosc.2012.09.010. Epub 2012 Oct 12.

Authors

Damien Michelon¹, Sybille Tachon, Bruno Ebel, Joëlle De Coninck, Gilles Feron, Patrick Gervais, Mireille Yvon, Rémy Cachon

Affiliation

¹ UMR A 02.102 Unité Procédés Alimentaires et Microbiologiques, AgroSup Dijon-Université de Bourgogne, 1-esplanade Erasme, F-21000 Dijon, France.

PMID: 23063698
DOI: 10.1016/j.jbiosc.2012.09.010

Abstract

Reducing activity is a physiological property of lactic acid bacteria (LAB) of technological importance. We developed a solid medium with tetrazolium dyes enabling weakly and strongly reducing LAB to be discriminated. It was used to quantify populations in a mixed culture (spreading method) and screen strains (spot method).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar / chemistry
Agar / metabolism*
Animals
Culture Media / chemistry
Culture Media / metabolism
Lactic Acid / metabolism*
Lactobacillaceae / classification
Lactobacillaceae / growth & development
Lactobacillaceae / isolation & purification*
Lactobacillaceae / metabolism*
Milk / metabolism*
Milk / microbiology*
Oxidation-Reduction
Tetrazolium Salts / chemistry

Substances

Culture Media
Tetrazolium Salts
Lactic Acid
Agar