Abstract
The precise mechanism of characteristic Th2 predominance at maternal-fetal interface remains unresolved. In the present study, we investigated roles of the decidua-derived CCL2 in Th2 predominance at maternal-fetal interface. FCM shows that 55% CD56(+)CD16(-)CD3(-) decidual NK, 52% CD4(+) T cells and 75% CD14(+) monocytes express CCR2. Recombinant human CCL2 (rhCCL2) and the decidual stromal cells (DSCs)-derived supernatant can enhance proliferation and inhibit apoptosis of these decidual leukocytes (DLCs), and promote Th2 cytokines production, IL-4 and IL-10, with an increase in GATA-3 transcription. They also inhibit the secretion of Th1 cytokines, TNF-α and IFN-γ, with a decrease in T-bet transcription It is concluded that the secreted CCL2 by decidual stromal cells increases GATA-3 transcription and decreases T-bet transcription in the decidual leukocytes, which contributes to Th2 polarization at maternal-fetal interface. Furthermore, the Th2 cytokines, IL-4 and IL-10, rather than Th1 cytokines, was shown to increase CCL2 secretion of DSC.
Copyright © 2012 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Antigens, CD / genetics
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Antigens, CD / immunology
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Cell Differentiation / drug effects
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Cell Differentiation / immunology
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Cell Proliferation / drug effects
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Chemokine CCL2 / immunology*
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Chemokine CCL2 / metabolism
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Chemokine CCL2 / pharmacology
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Culture Media, Conditioned
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Decidua / cytology
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Decidua / drug effects*
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Decidua / immunology
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Female
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GATA3 Transcription Factor / agonists
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GATA3 Transcription Factor / genetics
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GATA3 Transcription Factor / metabolism
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Humans
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Killer Cells, Natural / cytology
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology*
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Monocytes / cytology
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Monocytes / drug effects
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Monocytes / immunology*
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Pregnancy / immunology*
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Pregnancy Trimester, First
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Receptors, CCR2 / genetics
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Receptors, CCR2 / immunology
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Signal Transduction
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Stromal Cells / cytology
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Stromal Cells / immunology
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Stromal Cells / metabolism*
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Th1-Th2 Balance / drug effects
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Th2 Cells / cytology
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Th2 Cells / drug effects
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Th2 Cells / immunology*
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Transcription, Genetic
Substances
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Antigens, CD
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CCL2 protein, human
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CCR2 protein, human
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Chemokine CCL2
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Culture Media, Conditioned
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GATA3 Transcription Factor
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Receptors, CCR2
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Recombinant Proteins