Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships

Microscopy (Oxf). 2013 Apr;62(2):317-20. doi: 10.1093/jmicro/dfs069. Epub 2012 Oct 18.

Abstract

To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / pathology*
  • Cell Communication
  • Dendritic Cells / physiology
  • Dendritic Cells / ultrastructure*
  • Humans
  • Image Processing, Computer-Assisted
  • Imaging, Three-Dimensional
  • Microscopy, Electron, Scanning
  • Plasma Cells / physiology
  • Plasma Cells / ultrastructure*
  • Synovial Membrane / ultrastructure*
  • Tomography, X-Ray Computed