Use of dried blood spots for HIV-1 genotyping in Southeast Asia: Thailand experience

Wiriya Rutvisuttinunt; Miguel A Arroyo; Vatcharain Assawadarachai; Kultida Poltavee; Francine E McCutchan; Sodsai Tovanabutra; Gustavo Kijak; Jerome H Kim; Robert M Paris; Mark de Souza

Use of dried blood spots for HIV-1 genotyping in Southeast Asia: Thailand experience

Southeast Asian J Trop Med Public Health. 2012 Mar;43(2):333-9.

Authors

Wiriya Rutvisuttinunt¹, Miguel A Arroyo, Vatcharain Assawadarachai, Kultida Poltavee, Francine E McCutchan, Sodsai Tovanabutra, Gustavo Kijak, Jerome H Kim, Robert M Paris, Mark de Souza

Affiliation

¹ Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. [email protected]

PMID: 23082585

Abstract

The multi-region hybridization assay (MHAbce) for genotyping HIV-1 subtypes B, C and circulating recombinant form (CRF01_AE) was evaluated on paired plasma and dried blood spots (DBS) collected from 68 HIV-1 infected individuals in Thailand. CRF01_AE was the predominant subtype identified using plasma samples (51/62) and DBS (24/27). There was no discordance in subtype designations between plasma and DBS.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Genotype
HIV Seropositivity / epidemiology
HIV Seropositivity / genetics*
HIV-1 / genetics*
Humans
Molecular Epidemiology / methods*
Sensitivity and Specificity
Thailand / epidemiology
Viral Load