Complete nucleotide sequence of the first KPC-2- and SHV-12-encoding IncX plasmid, pKpS90, from Klebsiella pneumoniae

Antimicrob Agents Chemother. 2013 Jan;57(1):618-20. doi: 10.1128/AAC.01712-12. Epub 2012 Oct 22.

Abstract

We report the complete nucleotide sequence of the pKpS90 plasmid, carrying the bla(KPC-2) and bla(SHV-12) genes. This plasmid was isolated from a sequence type 258 (ST258) Klebsiella pneumoniae strain responsible for an outbreak in a French university hospital in 2009. pKpS90 is a 53,286-bp plasmid that belongs to the IncX incompatibility group. pKpS90 consists of a backbone from IncX plasmids, in which the KPC-2-encoding Tn4401 transposon and a bla(SHV-12)-encoding region have been inserted.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use*
  • Base Sequence
  • DNA Transposable Elements
  • Disease Outbreaks*
  • France / epidemiology
  • Hospitals, University
  • Humans
  • Klebsiella Infections / drug therapy
  • Klebsiella Infections / epidemiology*
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids*
  • Sequence Analysis, DNA
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactams / pharmacology
  • beta-Lactams / therapeutic use*

Substances

  • Anti-Bacterial Agents
  • DNA Transposable Elements
  • beta-Lactams
  • beta-lactamase SHV-12
  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae

Associated data

  • GENBANK/JX461340