Transgenic expression of the Helicobacter pylori virulence factor CagA promotes apoptosis or tumorigenesis through JNK activation in Drosophila

PLoS Pathog. 2012;8(10):e1002939. doi: 10.1371/journal.ppat.1002939. Epub 2012 Oct 18.

Abstract

Gastric cancer development is strongly correlated with infection by Helicobacter pylori possessing the effector protein CagA. Using a transgenic Drosophila melanogaster model, we show that CagA expression in the simple model epithelium of the larval wing imaginal disc causes dramatic tissue perturbations and apoptosis when CagA-expressing and non-expressing cells are juxtaposed. This cell death phenotype occurs through activation of JNK signaling and is enhanced by loss of the neoplastic tumor suppressors in CagA-expressing cells or loss of the TNF homolog Eiger in wild type neighboring cells. We further explored the effects of CagA-mediated JNK pathway activation on an epithelium in the context of oncogenic Ras activation, using a Drosophila model of metastasis. In this model, CagA expression in epithelial cells enhances the growth and invasion of tumors in a JNK-dependent manner. These data suggest a potential role for CagA-mediated JNK pathway activation in promoting gastric cancer progression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / metabolism*
  • Apoptosis*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cell Transformation, Neoplastic*
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / genetics
  • Helicobacter pylori / genetics*
  • Helicobacter pylori / metabolism
  • Helicobacter pylori / pathogenicity
  • Imaginal Discs / metabolism
  • JNK Mitogen-Activated Protein Kinases / metabolism*
  • MAP Kinase Signaling System
  • Membrane Proteins / genetics*
  • Neoplasm Metastasis
  • Oncogene Protein p21(ras) / metabolism
  • Signal Transduction
  • Wings, Animal

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Drosophila Proteins
  • Membrane Proteins
  • cagA protein, Helicobacter pylori
  • egr protein, Drosophila
  • JNK Mitogen-Activated Protein Kinases
  • Oncogene Protein p21(ras)