Humanized mouse model of skin inflammation is characterized by disturbed keratinocyte differentiation and influx of IL-17A producing T cells

PLoS One. 2012;7(10):e45509. doi: 10.1371/journal.pone.0045509. Epub 2012 Oct 19.

Abstract

Humanized mouse models offer a challenging possibility to study human cell function in vivo. In the huPBL-SCID-huSkin allograft model human skin is transplanted onto immunodeficient mice and allowed to heal. Thereafter allogeneic human peripheral blood mononuclear cells are infused intra peritoneally to induce T cell mediated inflammation and microvessel destruction of the human skin. This model has great potential for in vivo study of human immune cells in (skin) inflammatory processes and for preclinical screening of systemically administered immunomodulating agents. Here we studied the inflammatory skin response of human keratinocytes and human T cells and the concomitant systemic human T cell response.As new findings in the inflamed human skin of the huPBL-SCID-huSkin model we here identified: 1. Parameters of dermal pathology that enable precise quantification of the local skin inflammatory response exemplified by acanthosis, increased expression of human β-defensin-2, Elafin, K16, Ki67 and reduced expression of K10 by microscopy and immunohistochemistry. 2. Induction of human cytokines and chemokines using quantitative real-time PCR. 3. Influx of inflammation associated IL-17A-producing human CD4+ and CD8+ T cells as well as immunoregulatory CD4+Foxp3+ cells using immunohistochemistry and -fluorescence, suggesting that active immune regulation is taking place locally in the inflamed skin. 4. Systemic responses that revealed activated and proliferating human CD4+ and CD8+ T cells that acquired homing marker expression of CD62L and CLA. Finally, we demonstrated the value of the newly identified parameters by showing significant changes upon systemic treatment with the T cell inhibitory agents cyclosporine-A and rapamycin. In summary, here we equipped the huPBL-SCID-huSkin humanized mouse model with relevant tools not only to quantify the inflammatory dermal response, but also to monitor the peripheral immune status. This combined approach will gain our understanding of the dermal immunopathology in humans and benefit the development of novel therapeutics for controlling inflammatory skin diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / pathology
  • CD4-Positive T-Lymphocytes / transplantation
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / pathology
  • CD8-Positive T-Lymphocytes / transplantation
  • Cell Differentiation
  • Cyclosporine / pharmacology
  • Disease Models, Animal*
  • Elafin / genetics
  • Elafin / immunology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Humans
  • Inflammation / drug therapy
  • Inflammation / immunology*
  • Inflammation / pathology
  • Injections, Intraperitoneal
  • Interleukin-17 / biosynthesis*
  • Interleukin-17 / immunology
  • Keratinocytes / drug effects
  • Keratinocytes / immunology*
  • Keratinocytes / pathology
  • Keratins / genetics
  • Keratins / immunology
  • Ki-67 Antigen / genetics
  • Ki-67 Antigen / immunology
  • L-Selectin / genetics
  • L-Selectin / immunology
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, SCID
  • Sirolimus / pharmacology
  • Skin / drug effects
  • Skin / immunology*
  • Skin / pathology
  • Skin Transplantation*
  • Transplantation, Heterologous
  • beta-Defensins / genetics
  • beta-Defensins / immunology

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CTAGE1 protein, human
  • DEFB4A protein, human
  • Elafin
  • IL17A protein, human
  • Interleukin-17
  • Ki-67 Antigen
  • Membrane Glycoproteins
  • PI3 protein, human
  • beta-Defensins
  • L-Selectin
  • Keratins
  • Cyclosporine
  • Sirolimus

Grants and funding

Part of this work was supported by a research grant from the VanderEs Research Foundation and other part was supported by a research grant from LEO Pharma A/S. The funders had no role in study design, data collection, decision to publish, or preparation of the manuscript. However, they have been involved in a few sample analyses and data discussions in addition to manuscript reviewing.