Green tea polyphenol protection against 4-nitroquinoline 1-oxide-induced bone marrow lipid peroxidation and genotoxicity in Wistar rats

Asian Pac J Cancer Prev. 2012;13(8):4107-12. doi: 10.7314/apjcp.2012.13.8.4107.

Abstract

4-Nitroquinoline 1-oxide (4-NQO) a potent oral carcinogen, widely used for induction of oral carcinogenesis, has been found to induce lipid peroxidation in vivo and in vitro. Green tea contains a high content of polyphenols, which are potent antioxidants. Thus green tea polyphenols (GTP) might be expected play a protective role against 4-NQO induced lipid peroxidation and bone marrow toxicity. In the present study, a dose of 200 mg of GTP/kg b.wt/day was given orally for a week, simultaneously animals received 0.2 ml of 0.5% 4-NQO in propylene glycol (5 mg/ml) injected intramuscularly for three times/week. Oxidants and antioxidants such as malendialdehyde (MDA) and thiols, glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) were significantly decreased in 4-NQO induced animals except MDA, and these parameters were brought back to near normalcy on treatment with GTP. The results suggest that GTP treatment offers significant protection against 4-NQO induced lipid peroxidation and bone marrow toxicity and might be a promising potential candidate for prevention of mutations leading to cancer.

MeSH terms

  • 4-Nitroquinoline-1-oxide / toxicity*
  • Animals
  • Bone Marrow / drug effects*
  • Bone Marrow / pathology
  • Carcinogens / toxicity*
  • Catalase / metabolism
  • DNA Adducts / drug effects
  • DNA Damage / drug effects*
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Immunoenzyme Techniques
  • Lipid Peroxidation / drug effects*
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Polyphenols / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Tea / chemistry*

Substances

  • Carcinogens
  • DNA Adducts
  • Polyphenols
  • Tea
  • Malondialdehyde
  • 4-Nitroquinoline-1-oxide
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Glutathione