Mechanistic and structural insight into the functional dichotomy between IL-2 and IL-15

Nat Immunol. 2012 Dec;13(12):1187-95. doi: 10.1038/ni.2449. Epub 2012 Oct 28.

Abstract

Interleukin 15 (IL-15) and IL-2 have distinct immunological functions even though both signal through the receptor subunit IL-2Rβ and the common γ-chain (γ(c)). Here we found that in the structure of the IL-15-IL-15Rα-IL-2Rβ-γ(c) quaternary complex, IL-15 binds to IL-2Rβ and γ(c) in a heterodimer nearly indistinguishable from that of the IL-2-IL-2Rα-IL-2Rβ-γ(c) complex, despite their different receptor-binding chemistries. IL-15Rα substantially increased the affinity of IL-15 for IL-2Rβ, and this allostery was required for IL-15 trans signaling. Consistent with their identical IL-2Rβ-γ(c) dimer geometries, IL-2 and IL-15 showed similar signaling properties in lymphocytes, with any differences resulting from disparate receptor affinities. Thus, IL-15 and IL-2 induced similar signals, and the cytokine specificity of IL-2Rα versus IL-15Rα determined cellular responsiveness. Our results provide new insights for the development of specific immunotherapeutics based on IL-15 or IL-2.

Publication types

  • Research Support, American Recovery and Reinvestment Act
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Cell Line, Tumor
  • Crystallography, X-Ray
  • Humans
  • Interleukin-15 / chemistry
  • Interleukin-15 / immunology*
  • Interleukin-15 / metabolism
  • Interleukin-2 / chemistry
  • Interleukin-2 / immunology*
  • Interleukin-2 / metabolism
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Interleukin-2 Receptor beta Subunit / metabolism
  • Ligands
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Mice
  • Models, Molecular
  • Molecular Dynamics Simulation
  • Protein Binding
  • Protein Multimerization
  • Protein Structure, Quaternary
  • Signal Transduction

Substances

  • Interleukin-15
  • Interleukin-2
  • Interleukin-2 Receptor alpha Subunit
  • Interleukin-2 Receptor beta Subunit
  • Ligands

Associated data

  • GEO/GSE40350
  • PDB/4GS7