Comparing beneficial effects of inhaled nitric oxide to L-arginine in necrotizing enterocolitis model in neonatal rats

Pediatr Surg Int. 2012 Dec;28(12):1219-24. doi: 10.1007/s00383-012-3181-8. Epub 2012 Oct 30.

Abstract

Objective: Necrotizing enterocolitis (NEC) is a common and devastating gastrointestinal condition of neonatal infants. The pathophysiology of NEC remains poorly understood. We tried to evaluate the effectiveness of inhaled NO compared to L-arginine usage in necrotizing enterocolitis model in rats.

Material-methods: 46 newborn pups from 4 time-mated Sprague-Dawley pregnant rats were divided equally into 4 groups as follows: NEC (subjected to NEC), NEC + L-arginine, NEC + inhaled NO and control.

Results: SOD, GSH-Px and NOx levels were significantly higher and MDA levels were significantly lower in NEC + inhaled NO group compared to NEC + L-arginine group. There was significantly lower intestinal injury and apoptosis index scoring in NEC + inhaled NO group compared to NEC + L-arginine group.

Conclusion: We think that inhaled NO can be used as a novel therapeutic agent like L-arginine in NEC, like using in pulmonary hypertention in newborns but much more studies are needed.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Inhalation
  • Animals
  • Animals, Newborn
  • Arginine / therapeutic use*
  • Disease Models, Animal
  • Enterocolitis, Necrotizing / drug therapy*
  • Nitric Oxide / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Nitric Oxide
  • Arginine