Objective: To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province.
Methods: Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences.
Results: Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences. In total 7 genera and 22 species were identified, including 46 strains in Gammaproteobacteria (51.1%), 32 in Firmicutes (35.6%), 10 in Betaproteobacteria (11.1%), and 2 in Alphaproteobacteria (2.2%). The dominant bacteria species were Pseudomonas and Bacillus.
Conclusion: There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang, and these bacteria may play a physical and ecological roles in nematodes.