Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria

J Clin Microbiol. 1990 Feb;28(2):319-23. doi: 10.1128/jcm.28.2.319-323.1990.

Abstract

Oligodeoxynucleotide probes were developed for identification of the periodontal bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius types I and II, B. forsythus, Eikenella corrodens, Fusobacterium nucleatum, Haemophilus aphrophilus, Streptococcus intermedius, and Wolinella recta. Probes were designed by sequencing the 16S rRNA for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. These probes were specific when tested against a panel of nucleic acids from closely related bacteria.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Base Sequence
  • DNA Probes*
  • Dental Plaque / microbiology
  • Humans
  • Molecular Sequence Data
  • Mouth / microbiology
  • Periodontal Diseases / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Species Specificity

Substances

  • DNA Probes
  • RNA, Ribosomal, 16S