Thrombosis heralding aneurysmal rupture: an exploration of potential mechanisms in a novel giant swine aneurysm model

AJNR Am J Neuroradiol. 2013 Feb;34(2):346-53. doi: 10.3174/ajnr.A3407. Epub 2012 Nov 15.

Abstract

Background and purpose: The relationship between aneurysm dimensions, flow, thrombosis, and rupture remains poorly understood. We attempted to clarify this relationship by exploring various swine aneurysm models.

Materials and methods: Bilateral carotid aneurysms were constructed according to 3 protocols in 24 animals: small aneurysms with wide necks (group 1; n = 6 animals); small aneurysms with small necks (group 2; n = 4 animals), and giant aneurysms with large necks (group 3; n = 14 animals). Group 3 included 3 subgroups, related to testing the model in various experimental conditions: The neck was clipped in 3 animals; venous pouches lacked an endothelial lining in 4 animals; and 7 were control animals. Animals were followed until rupture, or for 1-4 weeks. Angiography was performed postoperatively and before euthanasia. We studied lesion pathology, paying attention to thrombosis, recanalization, wall composition, and perianeurysmal hemorrhage.

Results: Groups differed significantly in aneurysm dimensions and aspect ratio (P = .002). Ruptures occurred more frequently in animals with untreated giant aneurysms (7/7) than in animals with small wide-neck (0/6) or small-neck (2/4) aneurysms (P = .002). Ruptures occurred only in animals with thrombosed aneurysms. Lesions lacking an endothelial lining and 5 of 6 clipped venous pouches thrombosed but did not rupture. One giant lesion ruptured despite complete clipping. The wall was deficient in α-actin and was infiltrated with inflammatory cells and erythrocytes in all thrombosed cases, ruptured or not. Ruptures were associated with recanalizing channels in 9 of 10 cases.

Conclusions: Thrombosis, inflammation, and recanalization may precipitate aneurysmal ruptures in a swine model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aneurysm, Ruptured / complications
  • Aneurysm, Ruptured / pathology
  • Aneurysm, Ruptured / physiopathology*
  • Animals
  • Biopsy
  • Carotid Arteries / diagnostic imaging
  • Carotid Arteries / pathology
  • Carotid Artery Diseases / complications
  • Carotid Artery Diseases / pathology
  • Carotid Artery Diseases / physiopathology*
  • Cerebral Angiography
  • Cerebrovascular Circulation / physiology
  • Disease Models, Animal
  • Intracranial Aneurysm / complications
  • Intracranial Aneurysm / pathology
  • Intracranial Aneurysm / physiopathology*
  • Severity of Illness Index
  • Sus scrofa
  • Thrombosis / complications
  • Thrombosis / pathology
  • Thrombosis / physiopathology*
  • Vasculitis / complications
  • Vasculitis / pathology
  • Vasculitis / physiopathology