The O2, pH and Ca2+ microenvironment of benthic foraminifera in a high CO2 world

PLoS One. 2012;7(11):e50010. doi: 10.1371/journal.pone.0050010. Epub 2012 Nov 15.

Abstract

Ocean acidification (OA) can have adverse effects on marine calcifiers. Yet, phototrophic marine calcifiers elevate their external oxygen and pH microenvironment in daylight, through the uptake of dissolved inorganic carbon (DIC) by photosynthesis. We studied to which extent pH elevation within their microenvironments in daylight can counteract ambient seawater pH reductions, i.e. OA conditions. We measured the O(2) and pH microenvironment of four photosymbiotic and two symbiont-free benthic tropical foraminiferal species at three different OA treatments (~432, 1141 and 2151 µatm pCO(2)). The O(2) concentration difference between the seawater and the test surface (ΔO(2)) was taken as a measure for the photosynthetic rate. Our results showed that O(2) and pH levels were significantly higher on photosymbiotic foraminiferal surfaces in light than in dark conditions, and than on surfaces of symbiont-free foraminifera. Rates of photosynthesis at saturated light conditions did not change significantly between OA treatments (except in individuals that exhibited symbiont loss, i.e. bleaching, at elevated pCO(2)). The pH at the cell surface decreased during incubations at elevated pCO(2), also during light incubations. Photosynthesis increased the surface pH but this increase was insufficient to compensate for ambient seawater pH decreases. We thus conclude that photosynthesis does only partly protect symbiont bearing foraminifera against OA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Australia
  • Calcium / analysis*
  • Carbon Dioxide / analysis
  • Cellular Microenvironment*
  • Foraminifera / metabolism*
  • Foraminifera / physiology
  • Hydrogen-Ion Concentration
  • Microelectrodes
  • Oxygen / analysis*
  • Pacific Ocean
  • Photosynthesis / physiology*
  • Seawater / chemistry*
  • Species Specificity
  • Statistics, Nonparametric

Substances

  • Carbon Dioxide
  • Oxygen
  • Calcium

Grants and funding

This project was funded by the German BMBF Project BioAcid (03F0608C), the Max Planck Institute for Marine Microbiology (MPI), and the Australian Institute of Marine Science (AIMS), who are thanked for their continuous support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.