Microcirculation inflammation associates with outcome in renal transplant patients with de novo donor-specific antibodies

Am J Transplant. 2013 Feb;13(2):485-92. doi: 10.1111/j.1600-6143.2012.04325.x. Epub 2012 Nov 21.

Abstract

In renal transplant patients with de novo donor-specific antibodies (dnDSA) we studied the value of microcirculation inflammation (MI; defined by the addition of glomerulitis (g) and peritubular capillaritis (ptc) scores) to assess long-term graft survival in a retrospective cohort study. Out of all transplant patients with standard immunological risk (n = 638), 79 (12.4%) developed dnDSA and 58/79 (73%) had an indication biopsy at or after dnDSA development. Based on the MI score on that indication biopsy patients were categorized, MI0 (n = 26), MI1 + 2 (n = 21) and MI ≥ 3 (n = 11). The MI groups did not differ significantly pretransplantation, whereas posttransplantation higher MI scores developed more anti-HLA class I + II DSA (p = 0.011), showed more TCMR (p < 0.001) and showed a trend to C4d-positive staining (p = 0.059). Four-year graft survival estimates from time of indication biopsy were MI0 96.1%, MI1 + 2 76.1% and MI ≥ 3 17.1%; resulting in a 24-fold increased risk of graft failure in the MI ≥ 3 compared to the MI0 group (p = 0.003; 95% CI [3.0-196.0]). When adjusted for C4d, MI ≥ 3 still had a 21-fold increased risk of graft failure (p = 0.005; 95% CI [2.5-180.0]), while C4d positivity on indication biopsy lost significance. In renal transplant patients with de novo DSA, microcirculation inflammation, defined by g + ptc, associates with graft survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies / immunology*
  • Biopsy
  • Complement C4b / analysis
  • Female
  • Graft Rejection / immunology
  • Graft Survival
  • Humans
  • Immunosuppressive Agents / therapeutic use
  • Inflammation
  • Kidney / immunology*
  • Kidney Transplantation / immunology*
  • Kidney Transplantation / methods*
  • Male
  • Microcirculation
  • Middle Aged
  • Models, Statistical
  • Peptide Fragments / analysis
  • Renal Insufficiency / therapy*
  • Retrospective Studies
  • Risk Factors
  • Tissue Donors

Substances

  • Antibodies
  • Immunosuppressive Agents
  • Peptide Fragments
  • Complement C4b
  • complement C4d