Effect of high-dose or split-dose artesunate on parasite clearance in artemisinin-resistant falciparum malaria

Clin Infect Dis. 2013 Mar;56(5):e48-58. doi: 10.1093/cid/cis958. Epub 2012 Nov 21.

Abstract

Background: The emergence of Plasmodium falciparum resistance to artemisinins on the Cambodian and Myanmar-Thai borders poses severe threats to malaria control. We investigated whether increasing or splitting the dose of the short-half-life drug artesunate improves parasite clearance in falciparum malaria in the 2 regions.

Methods: In Pailin, western Cambodia (from 2008 to 2010), and Wang Pha, northwestern Thailand (2009-2010), patients with uncomplicated falciparum malaria were randomized to oral artesunate 6 mg/kg/d as a once-daily or twice-daily dose for 7 days, or artesunate 8 mg/kg/d as a once-daily or twice-daily dose for 3 days, followed by mefloquine. Parasite clearance and recrudescence for up to 63 days of follow-up were assessed.

Results: A total of 159 patients were enrolled. Overall median (interquartile range [IQR]) parasitemia half-life (half-life) was 6.03 (4.89-7.28) hours in Pailin versus 3.42 (2.20-4.85) hours in Wang Pha (P = .0001). Splitting or increasing the artesunate dose did not shorten half-life in either site. Pharmacokinetic profiles of artesunate and dihydroartemisinin were similar between sites and did not correlate with half-life. Recrudescent infections occurred in 4 of 79 patients in Pailin and 5 of 80 in Wang Pha and was not different between treatment arms (P = .68).

Conclusions: Increasing the artesunate treatment dose up to 8 mg/kg/d or splitting the dose does not improve parasite clearance in either artemisinin resistant or more sensitive infections with P. falciparum. Clinical Trials Registration. ISRCTN15351875.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Adolescent
  • Adult
  • Antibodies, Protozoan / blood
  • Antimalarials / administration & dosage*
  • Antimalarials / pharmacokinetics
  • Artemisinins / administration & dosage*
  • Artemisinins / pharmacokinetics
  • Artemisinins / therapeutic use*
  • Artesunate
  • Cambodia
  • Child
  • Drug Resistance*
  • Female
  • Half-Life
  • Humans
  • Immunoglobulin G / blood
  • Malaria, Falciparum / drug therapy*
  • Malaria, Falciparum / immunology
  • Male
  • Parasite Load
  • Parasitemia / drug therapy*
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / immunology
  • Thailand
  • Treatment Outcome
  • Young Adult

Substances

  • Antibodies, Protozoan
  • Antimalarials
  • Artemisinins
  • Immunoglobulin G
  • Artesunate
  • artemisinin