Methylation of the WNT5A gene is frequently detected in early gastric carcinoma

Hepatogastroenterology. 2012 Nov-Dec;59(120):2661-3. doi: 10.5754/hge09664.

Abstract

Background/aims: Recently, it has been reported that WNT5A methylation was frequently detected in colorectal cancers. However, the relationship between the WNT5A methylation and the characteristics of gastric cancer remains unknown.

Methodology: Methylation status of the WNT5A gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.

Results: Aberrant methylation of the WNT5A gene was detected in 7 out of the 38 (18%) primary gastric carcinomas, suggesting that the methylation of WNT5A is observed in gastric carcinomas as well as colorectal ones. The clinicopathological data were correlated with the methylation results. A significant difference was observed in the extent of tumor (p=0.0226). Moreover, a trend was shown towards early TNM stages in methylated tumors (p=0.209).

Conclusions: WNT5A was more frequently methylated in early gastric carcinomas.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Carcinoma / genetics*
  • Carcinoma / pathology
  • Chi-Square Distribution
  • DNA Methylation*
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Phenotype
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / genetics*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Wnt Proteins / genetics*
  • Wnt-5a Protein

Substances

  • Proto-Oncogene Proteins
  • WNT5A protein, human
  • Wnt Proteins
  • Wnt-5a Protein