VNN1 gene expression levels and the G-137T polymorphism are associated with HDL-C levels in Mexican prepubertal children

PLoS One. 2012;7(11):e49818. doi: 10.1371/journal.pone.0049818. Epub 2012 Nov 21.

Abstract

Background: VNN1 gene expression levels and the G-137T polymorphism have been associated with high density lipoprotein cholesterol (HDL-C) levels in Mexican American adults. We aim to evaluate the contribution of VNN1 gene expression and the G-137T variant to HDL-C levels and other metabolic traits in Mexican prepubertal children.

Methodology/principal findings: VNN1 mRNA expression levels were quantified in peripheral blood leukocytes from 224 unrelated Mexican-Mestizo children aged 6-8 years (107 boys and 117 girls) and were genotyped for the G-137T variant (rs4897612). To account for population stratification, a panel of 10 ancestry informative markers was analyzed. After adjustment for admixture, the TT genotype was significantly associated with lower VNN1 mRNA expression levels (P = 2.9 × 10(-5)), decreased HDL-C levels (β = -6.19, P = 0.028) and with higher body mass index (BMI) z-score (β = 0.48, P = 0.024) in the total sample. In addition, VNN1 expression showed a positive correlation with HDL-C levels (r = 0.220; P = 0.017) and a negative correlation with BMI z-score (r = -0.225; P = 0.015) only in girls.

Conclusion/significance: Our data suggest that VNN1 gene expression and the G-137T variant are associated with HDL-C levels in Mexican children, particularly in prepubertal girls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases* / blood
  • Amidohydrolases* / genetics
  • Body Mass Index
  • Child
  • Cholesterol, HDL* / blood
  • Cholesterol, HDL* / genetics
  • Female
  • GPI-Linked Proteins / blood
  • GPI-Linked Proteins / genetics
  • Gene Expression
  • Genotype
  • Humans
  • Male
  • Mexico
  • Polymorphism, Genetic*

Substances

  • Cholesterol, HDL
  • GPI-Linked Proteins
  • Amidohydrolases
  • pantetheinase

Grants and funding

This research was supported by grant 85010 from the Consejo Nacional de Ciencia y Tecnología (CONACyT, http://www.conacyt.mx/Paginas/default.aspx) and was partially supported by PAIP 4194-16. LJA is in the PhD Program from Ciencias Biomédicas at Universidad Nacional Autónoma de México (UNAM). LJA, HVR and PLM are recipients of the CONACyT scholarship number 195399, 244112 and 234714, respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.