Des-gamma-carboxy prothrombin identified by P-11 and P-16 antibodies reflects prognosis for patients with hepatocellular carcinoma

J Gastroenterol Hepatol. 2013 Apr;28(4):671-7. doi: 10.1111/jgh.12076.

Abstract

Background and aims: Serum des-γ-carboxy prothrombin (DCP) is an established tumor marker in patients with hepatocellular carcinoma (HCC), which can be identified by using MU-3 antibody. The MU-3 antibody mainly reacts with the 9-10 glutamic acid residues of DCP (conventional DCP). Since other variants of DCP with fewer glutamic acid residues can be detected using P-11 and P-16 antibodies (code name: NX-PVKA), we examined the clinical characteristics associated with NX-PVKA, and whether NX-PVKA is a useful measure in HCC patients.

Methods: Participants comprised 197 HCC patients admitted to our hospital between 2001 and 2010. NX-PVKA, conventional DCP, alpha-fetoprotein, and L3 fraction of alpha-fetoprotein were measured prior to initiation of HCC treatment.

Results: Of the tumor markers assessed, NX-PVKA was the only significant predictor of prognosis (hazard ratio, 81.32; P < 0.0001). Patients with NX-PVKA level ≥ 100 mAU/mL showed significantly lower survival rates (P < 0.0001). NX-PVKA level was also significantly associated with platelet count, prothrombin time, C-reactive protein, sex, maximum tumor size, number of nodules, and portal venous invasion by HCC. Finally, using NX-PVKA level and other clinical parameters, we established a prognostic model to estimate patient survival time.

Conclusions: NX-PVKA offers the best marker of tumor prognosis among HCC patients, and is strongly associated with tumor factors and hepatic functional reserve. NX-PVKA could be useful for clinical evaluation of tumor severity, as well as the estimated duration of survival among patients with HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Autoantibodies / blood*
  • Biomarkers
  • Biomarkers, Tumor / immunology*
  • Carcinoma, Hepatocellular / blood*
  • Carcinoma, Hepatocellular / mortality
  • Female
  • Humans
  • Liver Neoplasms / blood*
  • Liver Neoplasms / mortality
  • Luminescent Measurements
  • Male
  • Middle Aged
  • Prognosis
  • Proportional Hazards Models
  • Protein Precursors / immunology*
  • Prothrombin / immunology*
  • Retrospective Studies
  • Survival Rate

Substances

  • Autoantibodies
  • Biomarkers
  • Biomarkers, Tumor
  • Protein Precursors
  • acarboxyprothrombin
  • Prothrombin