Abstract
Aiming at identifying new scaffolds for BACE-1 inhibition devoid of the pharmacokinetic drawbacks of peptide-like structures, we investigated a series of novel peptidomimetics based on a 1,4-benzodiazepine (BDZ) core 1a-h and their seco-analogues 2a-d. We herein discuss synthesis, molecular modeling and in vitro studies which, starting from 1a, led to the seco-analogues (R)-2c and (S)-2d endowed with BACE-1 inhibition properties in the micromolar range both on the isolated enzyme and in cellular studies. These data can encourage to pursue these analogues as hits for the development of a new series of BACE-1 inhibitors active on whole-cells.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid Precursor Protein Secretases / antagonists & inhibitors*
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Amyloid Precursor Protein Secretases / genetics
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Amyloid Precursor Protein Secretases / metabolism
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Benzodiazepines / chemistry
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Binding Sites
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Catalytic Domain
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HEK293 Cells
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Humans
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Models, Chemical*
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Molecular Docking Simulation
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Peptidomimetics / chemical synthesis
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Peptidomimetics / chemistry*
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Peptidomimetics / metabolism
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Protease Inhibitors / chemical synthesis
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Protease Inhibitors / chemistry*
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Protease Inhibitors / metabolism
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Protein Binding
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Stereoisomerism
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Structure-Activity Relationship
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Transfection
Substances
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Peptidomimetics
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Protease Inhibitors
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Benzodiazepines
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Amyloid Precursor Protein Secretases