Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonicigenium vulgare

Yang Zou; Menglong Hu; Yajin Lv; Yang Wang; Hao Song; Ying-Jin Yuan

doi:10.1016/j.biortech.2012.10.151

Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonicigenium vulgare

Bioresour Technol. 2013 Mar:132:370-3. doi: 10.1016/j.biortech.2012.10.151. Epub 2012 Nov 7.

Authors

Yang Zou¹, Menglong Hu, Yajin Lv, Yang Wang, Hao Song, Ying-Jin Yuan

Affiliation

¹ Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.

PMID: 23218663
DOI: 10.1016/j.biortech.2012.10.151

Abstract

The co-cultures of Ketogulonicigenium vulgare and Bacillus cereus were subcultured daily for a total of 150 transfers. The yield of 2-keto-gulonic acid (2-KGA) and medium pH in the co-cultures were measured. We found that the serial subcultivation increased the yield of 2-KGA from 77% (original co-culture) to 93% (the 150th transfer of transferred co-culture). The resulted strains are of industrial interests for vitamin C production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascorbic Acid / biosynthesis
Bacillus cereus / growth & development
Bacillus cereus / metabolism*
Biotechnology / methods*
Cell Culture Techniques / methods*
Fermentation
Rhodobacteraceae / growth & development
Rhodobacteraceae / metabolism*
Sorbose / analysis
Sugar Acids / analysis
Sugar Acids / metabolism*

Substances

Sugar Acids
gulonic acid
Sorbose
Ascorbic Acid