Abstract
Bovine transforming growth factor-alpha (bTGF-alpha) is a 50 amino acid polypeptide with three disulfide linkages. In order to evaluate the biological function of this peptide, bTGF-alpha was synthesized via an automatic synthesizer and purified to homogeneity in high yield. The integrity of this synthetic peptide was confirmed by chemical analyses and bioassays. In a bovine liver radioreceptor assay, bTGF-alpha competes with radiolabeled EGF and has activity comparable to mEGF and hTGF-alpha. Compared to hEGF, bTGF-alpha elicits a greater response in a bovine mammary cell proliferation.
MeSH terms
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Amino Acid Sequence
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Animals
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Cattle
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Cell Division / drug effects
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Chromatography, High Pressure Liquid
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Epithelial Cells
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Epithelium / drug effects
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ErbB Receptors / drug effects
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ErbB Receptors / metabolism
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Humans
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Indicators and Reagents
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Kinetics
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Liver / metabolism
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Mammary Glands, Animal / cytology
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Mass Spectrometry
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Molecular Sequence Data
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Peptide Mapping
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Protein Conformation
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Rats
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Sequence Homology, Nucleic Acid
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Species Specificity
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Transforming Growth Factors / chemical synthesis*
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Transforming Growth Factors / pharmacology
Substances
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Indicators and Reagents
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Transforming Growth Factors
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ErbB Receptors