Abstract
2'-endo locked or frozen (S-type)/3'-endo locked or frozen (N-type) nucleoside analogues were synthesized. Conformational analysis based on (3)J(HH) and NOE measurements is presented which is further confirmed by X-ray crystal structural studies. 2'-5'isoDNA oligonucleotides (ON) were synthesized using these modified nucleoside analogues and UV-T(m) studies of the resultant 2'-5'isoDNA : RNA duplexes reflect the site- and sequence-dependent effects and confirm that the S-type sugar conformations were preferred over the N-type sugar geometry in such duplexes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Crystallography, X-Ray
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DNA / chemical synthesis
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DNA / chemistry
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DNA / metabolism
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Models, Molecular
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Molecular Conformation
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Nucleic Acid Conformation
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Nucleosides / chemical synthesis*
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Nucleosides / chemistry
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Nucleosides / metabolism
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Oligonucleotides / chemical synthesis
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Oligonucleotides / chemistry*
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Oligonucleotides / metabolism
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RNA / chemical synthesis
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RNA / chemistry*
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RNA / metabolism
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RNA Stability
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Ribonucleases / metabolism
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Snakes / metabolism
Substances
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Nucleosides
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Oligonucleotides
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locked nucleic acid
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RNA
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DNA
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Ribonucleases