[Cytokine determination from vitreous samples in retinal vascular diseases]

Ophthalmologe. 2013 Aug;110(8):746-54. doi: 10.1007/s00347-012-2719-4.
[Article in German]

Abstract

Purpose: The aim of this study was to determine cytokine levels from vitreous samples of treatment-naive patients with diabetic retinopathy (DRP), retinal vein occlusion (RVO) and exudative age-related macular degeneration (ARMD).

Methods: In this study 187 patients (median age 67 years, 101 males) were treated with a combined drug therapy including a 23-gauge core vitrectomy. Interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and intravitreal vascular endothelial growth factor (VEGF-A) levels were determined a using cytometric bead assay (CBA) and compared to those of the control group.

Results: Compared to the control group all diseases had significantly elevated cytokine levels, except VEGF in ARMD. In DRP samples of patients with diffuse diabetic macula edema (DME) higher VEGF-A and MCP-1 levels were found than in patients with focal DME. Ischemic DRP had higher VEGF levels than non-ischemic DRP. All measured cytokines were significantly higher in central retinal vein occlusion (CRVO) than in branch retinal vein occlusion (BRVO).

Conclusions: Differences in intravitreal cytokine levels in DRP, RVO and ARMD could be demonstrated. The knowledge of depicted specific characteristic dysregulation of cytokines could allow more targeted future therapies.

Publication types

  • English Abstract

MeSH terms

  • Aged
  • Biomarkers / analysis
  • Chemokine CCL2 / analysis*
  • Cytokines / analysis
  • Female
  • Germany / epidemiology
  • Humans
  • Interleukin-6 / analysis*
  • Male
  • Middle Aged
  • Prevalence
  • Reproducibility of Results
  • Retinal Diseases / diagnosis
  • Retinal Diseases / epidemiology*
  • Retinal Diseases / metabolism*
  • Retinal Vessels / chemistry*
  • Risk Factors
  • Sensitivity and Specificity
  • Vascular Endothelial Growth Factor A / analysis*
  • Vitreous Body / chemistry*

Substances

  • Biomarkers
  • CCL2 protein, human
  • Chemokine CCL2
  • Cytokines
  • Interleukin-6
  • Vascular Endothelial Growth Factor A