Abstract
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
MeSH terms
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Alphainfluenzavirus / enzymology
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Amantadine / pharmacology
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Antiviral Agents* / isolation & purification
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Antiviral Agents* / pharmacology
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Drug Evaluation, Preclinical / methods*
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Genes, Reporter*
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HEK293 Cells
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Humans
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Luciferases / genetics
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Luciferases / metabolism*
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Oseltamivir / pharmacology
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Plasmids
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RNA-Dependent RNA Polymerase / antagonists & inhibitors*
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RNA-Dependent RNA Polymerase / metabolism*
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Reproducibility of Results
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Ribavirin / pharmacology
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Sensitivity and Specificity
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Transfection
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Zanamivir / pharmacology
Substances
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Antiviral Agents
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Oseltamivir
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Ribavirin
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Amantadine
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Luciferases
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RNA-Dependent RNA Polymerase
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Zanamivir