Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury

Biomaterials. 2013 Mar;34(8):1987-94. doi: 10.1016/j.biomaterials.2012.11.040. Epub 2012 Dec 11.

Abstract

Vascular occlusion can result in fatal myocardial infarction, stroke or loss of limb in peripheral arterial disease. Interventional balloon angioplasty is a common first line procedure for vascular disease treatment, but long term success is limited by restenosis and neointimal hyperplasia. Cellular therapies have been proposed to mitigate these issues; however efficacy is low, in part due to poor cell retention. We show that magnetic targeting of mesenchymal stem cells gives rise to a 6-fold increase in cell retention following balloon angioplasty in a rabbit model using a clinically applicable permanent magnet. Cells labelled with superparamagnetic iron oxide nanoparticles exhibit no negative effects on cell viability, differentiation or secretion patterns. The increase in stem cell retention leads to a reduction in restenosis three weeks after cell delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis
  • Animals
  • Cell Adhesion
  • Chondrogenesis
  • Dextrans / therapeutic use*
  • Endothelium, Vascular / pathology
  • Femoral Artery / pathology
  • Graft Occlusion, Vascular / pathology
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Magnetic Resonance Spectroscopy
  • Magnetite Nanoparticles / therapeutic use*
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Microscopy, Confocal
  • Nanoparticles / therapeutic use*
  • Nanoparticles / ultrastructure
  • Osteogenesis
  • Rabbits
  • Staining and Labeling
  • Vascular System Injuries / pathology
  • Vascular System Injuries / surgery
  • Vascular System Injuries / therapy*

Substances

  • Dextrans
  • Intercellular Signaling Peptides and Proteins
  • Magnetite Nanoparticles
  • ferumoxides