Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines

FEBS Lett. 2013 Jan 16;587(2):231-7. doi: 10.1016/j.febslet.2012.12.002. Epub 2012 Dec 10.

Abstract

This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt's lymphoma cell lines, using 3'-phosphoadenosine 5'-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt's lymphoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anion Transport Proteins / antagonists & inhibitors
  • Anion Transport Proteins / genetics
  • Anion Transport Proteins / metabolism
  • Apoptosis / physiology
  • Apoptosis / radiation effects
  • Base Sequence
  • Burkitt Lymphoma / metabolism*
  • Burkitt Lymphoma / pathology
  • Burkitt Lymphoma / radiotherapy
  • Cell Line, Tumor
  • Humans
  • Keratan Sulfate / chemistry
  • Keratan Sulfate / metabolism*
  • MAP Kinase Signaling System
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism
  • Proteoglycans / chemistry
  • Proteoglycans / metabolism*
  • RNA, Small Interfering / genetics
  • Radiation Tolerance / physiology
  • Sulfate Transporters
  • Sulfuric Acid Esters / metabolism

Substances

  • Anion Transport Proteins
  • Membrane Transport Proteins
  • Proteoglycans
  • RNA, Small Interfering
  • SLC35B2 protein, human
  • SLC35B3 protein, human
  • Sulfate Transporters
  • Sulfuric Acid Esters
  • Keratan Sulfate