NK cytolysis is dependent on the proportion of HLA-G expression

Hum Immunol. 2013 Mar;74(3):286-9. doi: 10.1016/j.humimm.2012.12.005. Epub 2012 Dec 10.

Abstract

The suppressive functions of HLA-G to various immune cells have been well established. The proportion of HLA-G expression in malignant lesion cells was found from negative to 100%. However, effects for the different proportion of HLA-G expression on the cytolysis of NK cells remain to be explored. In this study, NK cytolysis to the various proportion of HLA-G1 expression on leukemia cell line K562 was investigated. Analysis of NK cell cytotoxicity was by detecting the NK cell surface CD107a expression. Data showed that NK cell cytolysis could be inhibited by the HLA-G1 expression and in a manner of HLA-G1 expression proportion dependent manner (r = 0.925, p = 0.008). Our study provided further understanding for the roles of HLA-G1 expression in malignant cell immune escaping from NK cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Coculture Techniques
  • Cytotoxicity, Immunologic / immunology*
  • Flow Cytometry
  • HLA-G Antigens / genetics
  • HLA-G Antigens / immunology*
  • HLA-G Antigens / metabolism
  • Humans
  • K562 Cells
  • Killer Cells, Natural / immunology*
  • Leukemia, Erythroblastic, Acute / genetics
  • Leukemia, Erythroblastic, Acute / immunology*
  • Leukemia, Erythroblastic, Acute / pathology
  • Transfection

Substances

  • HLA-G Antigens