Abstract
The v-src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF-IR gene (R-cells). R-/v-src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum-free condition. Using proteomic approaches on serum-free conditioned media derived from v-src-transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v-src-transformed cells in serum-deprived condition.
Copyright © 2012 Wiley Periodicals, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cell Transformation, Neoplastic / genetics*
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Cell Transformation, Neoplastic / metabolism*
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Culture Media, Serum-Free
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Gene Knockdown Techniques
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Genes, src*
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Glycoproteins / antagonists & inhibitors
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Glycoproteins / biosynthesis*
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Glycoproteins / genetics
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Intercellular Signaling Peptides and Proteins / biosynthesis*
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Intercellular Signaling Peptides and Proteins / genetics
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Mice
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Osteopontin / antagonists & inhibitors
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Osteopontin / biosynthesis*
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Osteopontin / genetics
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Prolactin
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Proteomics
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Receptor, IGF Type 1 / deficiency
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Receptor, IGF Type 1 / genetics
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Signal Transduction
Substances
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Culture Media, Serum-Free
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Glycoproteins
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Intercellular Signaling Peptides and Proteins
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Prl2c2 protein, mouse
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Spp1 protein, mouse
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Osteopontin
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Prolactin
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Receptor, IGF Type 1