Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts

Int J Biol Macromol. 2013 Mar:54:160-5. doi: 10.1016/j.ijbiomac.2012.12.009. Epub 2012 Dec 21.

Abstract

Carboxymethyl chitosan (CMC) was chemically modified by sulfating agent N(SO(3)Na)(3). The product carboxymethyl chitosan sulfate (CMCS) was characterized by Fourier transform infrared spectroscopy (FT-IR) and carbon-13 nuclear magnetic resonance spectroscopy ((13)C NMR). And the optimal reaction conditions were also studied by means of single factor experiment. MTT method was applied to evaluate the effects of CMCS on proliferation of skin fibroblasts. The results revealed the CMCS with sulfate content of 26.26% at the concentration of 100 μg/ml best promoted the proliferation of skin fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects
  • Cells, Cultured
  • Chitosan / analogs & derivatives*
  • Chitosan / chemical synthesis
  • Chitosan / chemistry
  • Chitosan / pharmacology
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Rats
  • Skin / cytology*
  • Spectroscopy, Fourier Transform Infrared
  • Sulfates / analysis
  • Temperature
  • Time Factors

Substances

  • Sulfates
  • carboxymethyl-chitosan
  • chitosan sulfate
  • Chitosan