Abstract
Abnormal migration of retinal pigment epithelium (RPE) contributes to a variety of disorders such as proliferative vitreoretinopathy. Here, the effect of epidermal growth factor (EGF), and signaling by its receptor (ERGR)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) on RPE cell migration was studied. The in vitro wound healing and migration of the human RPE cell line, ARPE19 cell was accelerated, in a dose dependent manner, in response to EGF stimulation, while pretreatment with EGFR, PI3K or AKT inhibitor, inhibited both events. Exposure of cells to EGF activated the AKT phosphorylation, whereas EGFR and PI3K inhibitors blocked EGF-induced AKT phosphorylation in a dose-dependent manner. These data suggest that EGF mediate ARPE-19 cell migration through EGFR/PI3K/AKT signaling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Growth Processes / physiology
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Cell Line
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Cell Movement / drug effects
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Cell Movement / physiology*
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Enzyme Inhibitors / pharmacology
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Epidermal Growth Factor / metabolism
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / metabolism*
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Humans
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Phosphatidylinositol 3-Kinase / metabolism*
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Phosphorylation / drug effects
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Proto-Oncogene Proteins c-akt / antagonists & inhibitors
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Proto-Oncogene Proteins c-akt / metabolism*
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Quinazolines / pharmacology
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Retinal Pigment Epithelium / cytology*
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Retinal Pigment Epithelium / drug effects
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Retinal Pigment Epithelium / enzymology*
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Retinaldehyde / metabolism
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Retinaldehyde / pharmacology
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Signal Transduction / drug effects
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Tyrphostins / pharmacology
Substances
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Enzyme Inhibitors
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Quinazolines
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Tyrphostins
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RTKI cpd
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Epidermal Growth Factor
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Phosphatidylinositol 3-Kinase
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ErbB Receptors
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Proto-Oncogene Proteins c-akt
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Retinaldehyde