Enhancement of the immunogenicity and protective efficacy of a mucosal influenza subunit vaccine by the saponin adjuvant GPI-0100

Heng Liu; Harshad P Patil; Jacqueline de Vries-Idema; Jan Wilschut; Anke Huckriede

doi:10.1371/journal.pone.0052135

Enhancement of the immunogenicity and protective efficacy of a mucosal influenza subunit vaccine by the saponin adjuvant GPI-0100

PLoS One. 2012;7(12):e52135. doi: 10.1371/journal.pone.0052135. Epub 2012 Dec 17.

Authors

Heng Liu¹, Harshad P Patil, Jacqueline de Vries-Idema, Jan Wilschut, Anke Huckriede

Affiliation

¹ Department of Medical Microbiology, Molecular Virology Section, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Abstract

Identification of safe and effective adjuvants remains an urgent need for the development of inactivated influenza vaccines for mucosal administration. Here, we used a murine challenge model to evaluate the adjuvant activity of GPI-0100, a saponin-derived adjuvant, on influenza subunit vaccine administered via the intranasal or the intrapulmonary route. Balb/c mice were immunized with 1 µg A/PR/8 (H1N1) subunit antigen alone or in combination with varying doses of GPI-0100. The addition of GPI-0100 was required for induction of mucosal and systemic antibody responses to intranasally administered influenza vaccine and significantly enhanced the immunogenicity of vaccine administered via the intrapulmonary route. Remarkably, GPI-0100-adjuvanted influenza vaccine given at a low dose of 2×1 µg either in the nares or directly into the lungs provided complete protection against homologous influenza virus infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic*
Administration, Intranasal
Animals
Antibodies, Viral / blood
Antibodies, Viral / immunology
Female
Immunoglobulin A, Secretory / immunology
Immunoglobulin G / blood
Immunoglobulin G / immunology
Influenza A Virus, H1N1 Subtype / immunology
Influenza Vaccines / administration & dosage
Influenza Vaccines / immunology*
Lung / immunology
Lung / pathology
Lung / virology
Mice
Mucous Membrane / immunology*
Orthomyxoviridae Infections / prevention & control
Orthomyxoviridae Infections / virology
Saponins / immunology*
T-Lymphocytes, Helper-Inducer / immunology
Vaccines, Subunit

Substances

Adjuvants, Immunologic
Antibodies, Viral
GPI0100
Immunoglobulin A, Secretory
Immunoglobulin G
Influenza Vaccines
Saponins
Vaccines, Subunit

Grants and funding

This study was conducted under the auspices of the Netherlands Influenza Vaccine Research Centre (NIVAREC), financially supported by the Netherlands Organisation for Health Research and Development (ZonMw). The study received a subsidy from Jan Kornelis de cock stichting (http://www.decockstichting.nl/subsidies.html). None of the funder had a role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.