Abstract
Acetylation of signal transducer and activator of transcription (STAT) proteins has been recognized as a significant mechanism for the regulation of their cellular functions. Site-specific antibodies are available only for a minority of STATs. The detection of acetylated STATs by immunoprecipitation (IP) followed by western blot (WB) will be described in the following chapter. Defined conditions for cell lysis and IP will be elucidated on the basis of STAT1 acetylation.
MeSH terms
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Acetylation
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Animals
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Blotting, Western / methods*
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Cell Extracts
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Cell Line
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Electrophoresis, Polyacrylamide Gel
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Humans
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Immunoprecipitation / methods*
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Lysine / metabolism
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Mice
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STAT Transcription Factors / chemistry
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STAT Transcription Factors / isolation & purification*
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STAT Transcription Factors / metabolism*
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STAT1 Transcription Factor / chemistry
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STAT1 Transcription Factor / isolation & purification
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STAT1 Transcription Factor / metabolism
Substances
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Cell Extracts
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STAT Transcription Factors
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STAT1 Transcription Factor
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Lysine