Immunosuppressive monoclonal antibody to CD64 from patients with long-term stable multiple sclerosis

J Neuroimmunol. 2013 Mar 15;256(1-2):62-70. doi: 10.1016/j.jneuroim.2012.12.007. Epub 2013 Jan 11.

Abstract

High intrathecal levels of anti-myelin basic protein (MBP) IgM were previously found to be significantly associated with early favorable course in a cohort of patients with multiple sclerosis (MS). A mAb to MBP 105-120 recognizing the 222-228 epitope of the extracellular domain of high affinity immunoglobulin gamma Fc-receptor I (CD64) was isolated from EBV(+) B cell clones of long-term stable RRMS patients. This mAb exerted immunosuppressive activity on MS-derived T cell lines through induction and release of high amounts of interleukin-10 and decreased levels of interleukin-12 from activated monocytes providing the biological basis for a potential new treatment for MS and other immune-mediated neurological disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal / blood
  • Antibodies, Monoclonal / pharmacology*
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Cell Line
  • Cell Proliferation / drug effects
  • Cohort Studies
  • Cytokines / genetics
  • Cytokines / metabolism
  • DNA-Binding Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Female
  • Flow Cytometry
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Immunoprecipitation
  • Immunosuppressive Agents / blood
  • Immunosuppressive Agents / pharmacology*
  • Male
  • Middle Aged
  • Multiple Sclerosis / pathology*
  • Myelin Basic Protein / immunology*
  • Myelin-Oligodendrocyte Glycoprotein / chemistry
  • Myelin-Oligodendrocyte Glycoprotein / immunology
  • Peptides / immunology
  • RNA, Messenger / metabolism
  • Receptors, IgG / genetics
  • Receptors, IgG / metabolism*
  • Statistics, Nonparametric
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Transcription Factors / immunology
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Cytokines
  • DNA-Binding Proteins
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunosuppressive Agents
  • MYT1 protein, human
  • Myelin Basic Protein
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptides
  • RNA, Messenger
  • Receptors, IgG
  • Transcription Factors