Adiponectin promotes functional recovery after podocyte ablation

J Am Soc Nephrol. 2013 Feb;24(2):268-82. doi: 10.1681/ASN.2012040414. Epub 2013 Jan 18.

Abstract

Low levels of the adipocyte-secreted protein adiponectin correlate with albuminuria in both mice and humans, but whether adiponectin has a causative role in modulating renal disease is unknown. Here, we first generated a mouse model that allows induction of caspase-8-mediated apoptosis specifically in podocytes upon injection of a construct-specific agent. These POD-ATTAC mice exhibited significant kidney damage, mimicking aspects of human renal disease, such as foot process effacement, mesangial expansion, and glomerulosclerosis. After the initial induction, both podocytes and filtration function recovered. Next, we crossed POD-ATTAC mice with mice lacking or overexpressing adiponectin. POD-ATTAC mice lacking adiponectin developed irreversible albuminuria and renal failure; conversely, POD-ATTAC mice overexpressing adiponectin recovered more rapidly and exhibited less interstitial fibrosis. In conclusion, these results suggest that adiponectin is a renoprotective protein after podocyte injury. Furthermore, the POD-ATTAC mouse provides a platform for further studies, allowing precise timing of podocyte injury and regeneration.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acetates / pharmacology
  • Adiponectin / genetics
  • Adiponectin / pharmacology*
  • Albuminuria / drug therapy
  • Albuminuria / pathology
  • Albuminuria / physiopathology
  • Animals
  • Apoptosis / physiology
  • Caspase 3 / metabolism
  • Caspase 8 / metabolism
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Female
  • Fibrosis / drug therapy
  • Fibrosis / pathology
  • Fibrosis / physiopathology
  • Glomerular Filtration Barrier / drug effects
  • Glomerular Filtration Barrier / pathology
  • Glomerular Filtration Barrier / physiology
  • Humans
  • Indoles / pharmacology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mice, Knockout
  • PPAR gamma / antagonists & inhibitors
  • Podocytes / pathology
  • Podocytes / physiology*
  • Recovery of Function / drug effects*
  • Recovery of Function / physiology*
  • Renal Insufficiency, Chronic / drug therapy*
  • Renal Insufficiency, Chronic / pathology
  • Renal Insufficiency, Chronic / physiopathology

Substances

  • 2-(2-(4-phenoxy-2-propylphenoxy)ethyl)indole-5-acetic acid
  • Acetates
  • Adiponectin
  • Adipoq protein, mouse
  • Indoles
  • PPAR gamma
  • Casp3 protein, mouse
  • Casp8 protein, mouse
  • Caspase 3
  • Caspase 8