A dual role of boronate affinity in high-sensitivity detection of vicinal diol brassinosteroids from sub-gram plant tissues via UPLC-MS/MS

Analyst. 2013 Mar 7;138(5):1342-5. doi: 10.1039/c3an36533f.

Abstract

Based on the dual role of specific boronate affinity, making use of both novel self-synthesized boronate affinity-functionalized magnetic nanoparticles and a high-efficiency organic boronic acid-type derivatization reagent, we report a simple, convenient and highly-sensitive method for detection of endogenous brassinosteroids from real plant materials.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boronic Acids / chemistry*
  • Brassinosteroids / analysis*
  • Chromatography, High Pressure Liquid / methods
  • Indicators and Reagents
  • Magnetite Nanoparticles / chemistry*
  • Plants / chemistry*
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry / methods*

Substances

  • Boronic Acids
  • Brassinosteroids
  • Indicators and Reagents
  • Magnetite Nanoparticles