Associations of in utero exposure to perfluorinated alkyl acids with human semen quality and reproductive hormones in adult men

Environ Health Perspect. 2013 Apr;121(4):453-8. doi: 10.1289/ehp.1205118. Epub 2013 Jan 28.

Abstract

Background: Perfluorinated alkyl acids (PFAAs), persistent chemicals with unique water-, dirt-, and oil-repellent properties, are suspected of having endocrine-disrupting activity. The PFAA compounds perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) are found globally in humans; because they readily cross the placental barrier, in utero exposure may be a cause for concern.

Objectives: We investigated whether in utero exposure to PFOA and PFOS affects semen quality, testicular volume, and reproductive hormone levels.

Methods: We recruited 169 male offspring (19-21 years of age) from a pregnancy cohort established in Aarhus, Denmark, in 1988-1989, corresponding to 37.6% of the eligible sons. Each man provided a semen sample and a blood sample. Semen samples were analyzed for sperm concentration, total sperm count, motility, and morphology, and blood samples were used to measure reproductive hormones. As a proxy for in utero exposure, PFOA and PFOS were measured in maternal blood samples from pregnancy week 30.

Results: Multivariable linear regression analysis suggested that in utero exposure to PFOA was associated with lower adjusted sperm concentration (ptrend = 0.01) and total sperm count (ptrend = 0.001) and with higher adjusted levels of luteinizing hormone (ptrend = 0.03) and follicle-stimulating hormone (ptrend = 0.01). PFOS did not appear to be associated with any of the outcomes assessed, before or after adjustment.

Conclusions: The results suggest that in utero exposure to PFOA may affect adult human male semen quality and reproductive hormone levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkanesulfonic Acids / blood*
  • Caprylates / blood*
  • Chromatography, Liquid
  • Cohort Studies
  • Denmark / epidemiology
  • Endocrine Disruptors / blood*
  • Environmental Exposure*
  • Female
  • Fluorocarbons / blood*
  • Gonadal Steroid Hormones / blood*
  • Humans
  • Linear Models
  • Luminescent Measurements
  • Male
  • Pregnancy
  • Prenatal Exposure Delayed Effects / chemically induced*
  • Prenatal Exposure Delayed Effects / epidemiology
  • Prospective Studies
  • Semen / drug effects*
  • Semen / physiology
  • Semen Analysis
  • Sperm Count
  • Tandem Mass Spectrometry
  • Testis / anatomy & histology
  • Testis / drug effects*
  • Young Adult

Substances

  • Alkanesulfonic Acids
  • Caprylates
  • Endocrine Disruptors
  • Fluorocarbons
  • Gonadal Steroid Hormones
  • perfluorooctanoic acid
  • perfluorooctane sulfonic acid