Effects by silodosin on the partially obstructed rat ureter in vivo and on human and rat isolated ureters

Br J Pharmacol. 2013 May;169(1):230-8. doi: 10.1111/bph.12123.

Abstract

Background and purpose: α1 -adrenoceptor (-AR) antagonists may facilitate ureter stone passage in humans. We aimed to study effects by the α1 A -AR selective antagonist silodosin (compared to tamsulosin and prazosin) on ureter pressures in a rat model of ureter obstruction, and on contractions of human and rat isolated ureters.

Experimental approach: After ethical approval, ureters of male rats were cannulated beneath the kidney pelvis for in vivo ureteral intraluminal recording of autonomous peristaltic pressure waves. A partial ureter obstruction was applied to the distal ureter. Mean arterial blood pressure (MAP) was recorded. Approximate clinical and triple clinical doses of the α1 -AR antagonists were given intravenously. Effects by the α1 -AR antagonists on isolated human and rat ureters were studied in organ baths.

Key results: Intravenous silodosin (0.1-0.3 mg kg(-1) ) or prazosin (0.03-0.1 mg kg(-1) ) reduced obstruction-induced increases in intraluminal ureter pressures by 21-37% or 18-40% respectively. Corresponding effects by tamsulosin (0.01 or 0.03 mg kg(-1) ) were 9-20%. Silodosin, prazosin and tamsulosin reduced MAP by 10-12%, 25-26% (P < 0.05), or 18-25% (P < 0.05) respectively. When effects by the α1 A -AR antagonists on obstruction-induced ureter pressures were expressed as a function of MAP, silodosin had six- to eightfold and 2.5- to eightfold better efficacy than tamsulosin or prazosin respectively. Silodosin effectively reduced contractions of both human and rat isolated ureters.

Conclusions and implications: Silodosin inhibits contractions of the rat and human isolated ureters and has excellent functional selectivity in vivo to relieve pressure-load of the rat obstructed ureter. Silodosin as pharmacological ureter stone expulsive therapy should be clinically further explored.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-1 Receptor Antagonists / administration & dosage
  • Adrenergic alpha-1 Receptor Antagonists / pharmacology
  • Animals
  • Arterial Pressure / drug effects
  • Dose-Response Relationship, Drug
  • Humans
  • Indoles / administration & dosage
  • Indoles / pharmacology*
  • Male
  • Muscle Contraction / drug effects
  • Prazosin / administration & dosage
  • Prazosin / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Species Specificity
  • Sulfonamides / administration & dosage
  • Sulfonamides / pharmacology
  • Tamsulosin
  • Ureter / drug effects*
  • Ureter / metabolism
  • Ureter / pathology
  • Ureteral Obstruction / drug therapy*
  • Ureteral Obstruction / pathology

Substances

  • Adrenergic alpha-1 Receptor Antagonists
  • Indoles
  • Sulfonamides
  • silodosin
  • Tamsulosin
  • Prazosin