Abstract
Recent reports in mice demonstrate that basophils function as antigen presenting cells (APC). They express MHC class II and co-stimulatory molecules CD80 and CD86, capture and present soluble antigens or IgE-antigen complexes and polarize Th2 responses. Therefore, we explored whether human circulating basophils possess the features of professional APC. We found that unlike dendritic cells (DC) and monocytes, steady-state circulating human basophils did not express HLA-DR and co-stimulatory molecules CD80 and CD86. Basophils remained negative for these molecules following stimulation with soluble Asp f 1, one of the allergens of Aspergillus fumigatus; Bet v 1, the major birch allergen; TLR2-ligand or even upon IgE cross-linking. Unlike DC, Asp f 1-pulsed basophils did not promote Th2 responses as analyzed by the secretion of IL-4 in the basophil-CD4(+) T cell co-culture. Together, these results demonstrate the inability of circulating human basophils to function as professional APC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens / genetics
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Allergens / metabolism
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Allergens / pharmacology
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Antigen-Presenting Cells / drug effects
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / metabolism*
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Antigens, Plant / genetics
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Antigens, Plant / metabolism
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Antigens, Plant / pharmacology
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B7-1 Antigen / metabolism
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B7-2 Antigen / metabolism
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Basophils / drug effects
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Basophils / immunology
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Basophils / metabolism*
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology
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Coculture Techniques
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Dendritic Cells / drug effects
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Fungal Proteins / pharmacology
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HLA-DR Antigens / metabolism
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Humans
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Interleukin-4 / metabolism
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Monocytes / drug effects
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Monocytes / immunology
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Monocytes / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
Substances
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ASPF1 protein, Aspergillus fumigatus
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Allergens
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Antigens, Plant
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B7-1 Antigen
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B7-2 Antigen
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Fungal Proteins
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HLA-DR Antigens
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Recombinant Proteins
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Bet v 1 allergen, Betula
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Interleukin-4