Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact

J Clin Microbiol. 2013 Apr;51(4):1301-2. doi: 10.1128/JCM.00028-13. Epub 2013 Feb 6.

Abstract

The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 94.8%; C. tropicalis, 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.

Publication types

  • Evaluation Study

MeSH terms

  • Candida / genetics
  • Candida / isolation & purification*
  • Candidemia / diagnosis*
  • Candidemia / microbiology
  • Clinical Laboratory Techniques / methods*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Diagnostic Techniques / methods*
  • Mycology / methods*
  • Sensitivity and Specificity