IgE production to α-gal is accompanied by elevated levels of specific IgG1 antibodies and low amounts of IgE to blood group B

PLoS One. 2013;8(2):e55566. doi: 10.1371/journal.pone.0055566. Epub 2013 Feb 4.

Abstract

IgE antibodies to gal-α-1,3-gal-β-1,4-GlcNAc (α-gal) can mediate a novel form of delayed anaphylaxis to red meat. Although IgG antibodies to α-gal (anti-α-gal or anti-Gal) are widely expressed in humans, IgE anti-α-gal is not. We explored the relationship between the IgG and IgE responses to both α-gal and the related blood group B antigen. Contradicting previous reports, antibodies to α-gal were found to be significantly less abundant in individuals with blood group B or AB. Importantly, we established a connection between IgE and IgG responses to α-gal: elevated titers of IgG anti-α-gal were found in IgE-positive subjects. In particular, proportionally more IgG1 anti-α-gal was found in IgE-positive subjects against a background of IgG2 production specific for α-gal. Thus, two types of immune response to α-gal epitopes can be distinguished: a 'typical' IgG2 response, presumably in response to gut bacteria, and an 'atypical', Th2-like response leading to IgG1 and IgE in addition to IgG2. These results suggest that IgE to a carbohydrate antigen can be formed (probably as part of a glycoprotein or glycolipid) even against a background of bacterial immune stimulation with essentially the same antigen.

MeSH terms

  • ABO Blood-Group System / blood
  • ABO Blood-Group System / immunology*
  • Anaphylaxis / blood
  • Anaphylaxis / immunology*
  • Animals
  • Carbohydrate Sequence
  • Case-Control Studies
  • Cattle
  • Epitopes / blood
  • Epitopes / immunology
  • Food Hypersensitivity / blood
  • Food Hypersensitivity / immunology*
  • Humans
  • Immunoconjugates / blood
  • Immunoconjugates / chemistry
  • Immunoconjugates / immunology
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology*
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology*
  • Meat Products*
  • Molecular Sequence Data
  • Radioimmunoassay
  • Serum Albumin / chemistry
  • Trisaccharides / blood
  • Trisaccharides / chemistry
  • Trisaccharides / immunology*

Substances

  • ABO Blood-Group System
  • Epitopes
  • Immunoconjugates
  • Immunoglobulin G
  • Serum Albumin
  • Trisaccharides
  • alpha-galactosyl epitope
  • Immunoglobulin E