Utility of OCT3/4, TSPY and β-catenin as biological markers for gonadoblastoma formation and malignant germ cell tumor development in dysgenetic gonads

Dis Markers. 2013;34(6):419-24. doi: 10.3233/DMA-130972.

Abstract

Background: Gonadoblastoma (GB) is regarded as an in situ form of germ cell tumor in dysgenetic gonads, and 30% of patients with GB develop a dysgerminoma/seminoma tumor.

Objective: Determine whether OCT3/4 and β-catenin are expressed in dysgenetic gonads before GB development and whether TSPY participates in the OCT3/4-β-catenin pathways in the malignant invasive behavior.

Methods: dysgenetic gonads of Disorders of sex differentiation (DSD) patients with mixed gonadal dysgenesis were analyzed by immunohistochemistry and immunofluorescence for comparison with GB and dysgerminoma/seminoma.

Results: Our results suggest that the development of GB is secondary to the interaction of OCT3/4 and TSPY, that β-catenin does not participate in this process.

Conclusions: The use of this biological markers detects the potential high risk gonads.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Case-Control Studies
  • Cell Cycle Proteins / analysis*
  • Child
  • Dysgerminoma / diagnosis
  • Gonadal Dysgenesis / diagnosis*
  • Gonadoblastoma / diagnosis*
  • Humans
  • Neoplasms, Gonadal Tissue / diagnosis*
  • Octamer Transcription Factor-3 / analysis*
  • beta Catenin / analysis*

Substances

  • Biomarkers, Tumor
  • CTNNB1 protein, human
  • Cell Cycle Proteins
  • Octamer Transcription Factor-3
  • POU5F1 protein, human
  • TSPY1 protein, human
  • beta Catenin