Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells

Gil Katz; Andrew L Snow

doi:10.1007/978-1-62703-290-2_2

Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells

Methods Mol Biol. 2013:979:15-23. doi: 10.1007/978-1-62703-290-2_2.

Authors

Gil Katz¹, Andrew L Snow

Affiliation

¹ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.

PMID: 23397384
DOI: 10.1007/978-1-62703-290-2_2

Abstract

After initial stimulation with antigen and exposure to the growth cytokine interleukin-2, activated T lymphocytes become sensitized to apoptosis upon antigen restimulation through the T cell receptor. This self-regulatory, restimulation-induced cell death (RICD) program constrains the proliferative capacity of activated T cells to help prevent excessive T cell accumulation and associated immunopathology. Here we describe a simple FACS-based approach for measuring RICD sensitivity in activated human T cells following polyclonal restimulation in vitro. This procedure is a straightforward research and clinical diagnostic tool for assessing RICD sensitivity for T cells derived from normal donors and patients suffering from diseases causing dysregulated T cell homeostasis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Culture Techniques
Flow Cytometry / methods*
Homeostasis
Humans
Lymphocyte Activation
Propidium / metabolism
Receptors, Antigen, T-Cell / metabolism*
T-Lymphocytes / cytology*
T-Lymphocytes / immunology
T-Lymphocytes / metabolism*

Substances

Receptors, Antigen, T-Cell
Propidium