Human immunodeficiency viruses: propagation, quantification, and storage

Curr Protoc Microbiol. 2013:Chapter 15:Unit 15J.1. doi: 10.1002/9780471729259.mc15j01s28.

Abstract

Described in this unit are basic protocols frequently used in the research of human immunodeficiency viruses (HIVs). Provided are methods for propagating and quantifying HIV, as well as recommendations for long-term storage. Background information about these methods is also provided and includes their advantages, disadvantages, and troubleshooting.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line
  • HIV-1 / growth & development*
  • HIV-1 / isolation & purification*
  • HIV-2 / growth & development*
  • HIV-2 / isolation & purification*
  • Humans
  • Preservation, Biological / methods*
  • Viral Load*
  • Virus Cultivation / methods