Caveolin-1 up-regulates integrin α2,6-sialylation to promote integrin α5β1-dependent hepatocarcinoma cell adhesion

Shengjin Yu; Jianhui Fan; Linhua Liu; Lijun Zhang; Shujing Wang; Jianing Zhang

doi:10.1016/j.febslet.2013.02.002

Caveolin-1 up-regulates integrin α2,6-sialylation to promote integrin α5β1-dependent hepatocarcinoma cell adhesion

FEBS Lett. 2013 Mar 18;587(6):782-7. doi: 10.1016/j.febslet.2013.02.002. Epub 2013 Feb 14.

Authors

Shengjin Yu¹, Jianhui Fan, Linhua Liu, Lijun Zhang, Shujing Wang, Jianing Zhang

Affiliation

¹ Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116044, Liaoning Province, China.

PMID: 23416306
DOI: 10.1016/j.febslet.2013.02.002

Abstract

The alterations of integrin glycosylation play a crucial role in tumor metastasis. Our previous studies indicated that caveolin-1 promoted the expression of the key α2,6-sialytransferase ST6Gal-I and fibronectin-mediated adhesion of mouse hepatocarcinoma cell. Herein, we investigated the role of α2,6-sialylated α5-integrin in the adhesion of mouse hepatocarcinoma H22 cell. We demonstrated that caveolin-1 up-regulated cell surface α2,6-linked sialic acid via stimulating ST6Gal-I transcription. Cell surface α2,6-sialylation was required for integrin α5β1-dependent cell adhesion to fibronectin, and an increase in α2,6-linked sialic acid on α5-subunit facilitated fibronectin-mediated focal adhesion kinase phosphorylations, suggesting that α2,6-sialylated α5-subunit promoted integrin α5β1-dependent cell adhesion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / pathology
Caveolin 1 / genetics*
Caveolin 1 / metabolism
Cell Adhesion
Cell Line, Tumor
Fibronectins / chemistry
Focal Adhesion Kinase 1 / genetics
Focal Adhesion Kinase 1 / metabolism
Gene Expression Regulation, Neoplastic
Genes, Reporter
Glycosylation
Integrin alpha2 / genetics*
Integrin alpha2 / metabolism
Integrin alpha5beta1 / genetics*
Integrin alpha5beta1 / metabolism
Integrin alpha6 / genetics*
Integrin alpha6 / metabolism
Liver Neoplasms / genetics*
Liver Neoplasms / metabolism
Liver Neoplasms / pathology
Luciferases
Mice
Phosphorylation
Sialic Acids / metabolism
Sialyltransferases / genetics*
Sialyltransferases / metabolism
Signal Transduction
Transcription, Genetic
beta-D-Galactoside alpha 2-6-Sialyltransferase

Substances

Caveolin 1
Fibronectins
Integrin alpha2
Integrin alpha5beta1
Integrin alpha6
Sialic Acids
Luciferases
Sialyltransferases
Focal Adhesion Kinase 1
Ptk2 protein, mouse
beta-D-Galactoside alpha 2-6-Sialyltransferase