Non-viral gene delivery via membrane-penetrating, mannose-targeting supramolecular self-assembled nanocomplexes

Adv Mater. 2013 Jun 11;25(22):3063-70. doi: 10.1002/adma.201205088. Epub 2013 Feb 18.

Abstract

Supramolecular self-assembled nanocomplexes (SSANs) capable of mannose receptor-mediated endocytosis and permeable to cellular and endosomal membranes are developed via the assembly of multiple rationally designed, function-specific materials. As a unique non-viral gene delivery vector, SSANs outperform commercial transfection reagents, including LPF2000, PEI, and jetPEI, by up to 2 orders of magnitude.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Membrane / metabolism*
  • Chitosan / chemistry
  • DNA / genetics
  • Drug Carriers / chemistry*
  • Drug Carriers / metabolism*
  • Drug Design
  • Hep G2 Cells
  • Humans
  • Luciferases / genetics
  • Mannose / metabolism*
  • Mice
  • Nanostructures / chemistry*
  • Plasmids / genetics
  • Polymers / chemistry
  • Transfection / methods*

Substances

  • Drug Carriers
  • Polymers
  • DNA
  • Chitosan
  • Luciferases
  • Mannose