Microarray analysis reveals novel features of the muscle aging process in men and women

J Gerontol A Biol Sci Med Sci. 2013 Sep;68(9):1035-44. doi: 10.1093/gerona/glt015. Epub 2013 Feb 15.

Abstract

To develop a global view of muscle transcriptional differences between older men and women and sex-specific aging, we obtained muscle biopsies from the biceps brachii of young and older men and women and profiled the whole-genome gene expression using microarray. A logistic regression-based method in combination with an intensity-based Bayesian moderated t test was used to identify significant sex- and aging-related gene functional groups. Our analysis revealed extensive sex differences in the muscle transcriptome of older individuals and different patterns of transcriptional changes with aging in men and women. In older women, we observed a coordinated transcriptional upregulation of immune activation, extracellular matrix remodeling, and lipids storage; and a downregulation of mitochondrial biogenesis and function and muscle regeneration. The effect of aging results in sexual dimorphic alterations in the skeletal muscle transcriptome, which may modify the risk for developing musculoskeletal and metabolic diseases in men and women.

Keywords: Aging; Sarcopenia; Sex; Transcription profile..

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aging / genetics*
  • Arm
  • Bayes Theorem
  • Female
  • Gene Expression Profiling
  • Humans
  • Male
  • Metabolic Diseases / etiology
  • Metabolic Diseases / genetics
  • Middle Aged
  • Muscle, Skeletal / metabolism*
  • Musculoskeletal Diseases / etiology
  • Musculoskeletal Diseases / genetics
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Risk Factors
  • Sex Characteristics
  • Transcriptome
  • Young Adult

Substances

  • RNA, Messenger